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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene is observed in several tumors, which leads to chromosome instability, and therefore might contribute to tumorigenesis. This protein was uncovered to affiliate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in cell-cycle controlled histone gene expression and performs a crucial role in endorsing cell-cycle progression from the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are already described. Two more splice variants had been reported but in-depth nucleotide sequence data is not still obtainable.
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Blocking peptides are peptides that bind specifically for the concentrate on antibody and block antibody binding. These peptide generally is made up of the epitope regarded from the antibody.
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This guideline outlines further more doable optimization of mobile and immunoassay parameters to be certain the best possible effects are...
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This guideline outlines even further probable optimization of cellular and immunoassay parameters to be sure the best possible benefits are...
Antibodies certain to the blocking peptide no more bind on the epitope about the focus on protein. By comparing the staining from your blocked antibody compared to the antibody alone, one can see which staining is unique.
Concentrate on Information and facts Cyclin E belongs to your remarkably conserved cyclin household, whose associates are characterized by a extraordinary periodicity in protein abundance from the mobile cycle. Cyclins purpose as regulators of CDK kinases. Diverse cyclins exhibit distinctive expression and degradation patterns which add into the temporal coordination of every mitotic celebration. Cyclin E forms a posh with and capabilities as a regulatory subunit of CDK2, whose action is needed for cell cycle G1/S changeover. This protein accumulates with the G1-S stage boundary and is particularly degraded as cells progress by S stage.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by the two antibodies...
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